header

AG Dr. Burkhard Krüger

Zellbiologie des exokrinen Pankreas

Methoden:

  • Primärzellkultur von Azini oder Azinuszellen aus dem Pankreas der Ratte 
  • in vitro Modulation spezifischer Zellaktivitäten durch Mikromanipulation und Mikroinjektion
  • Detektion von Enzymaktivitäten in lebenden Zellen mittels Spektrofluorimetrie und Mikrofluorimetrie
  • biochemische Analyse von Zellorganellen nach   Dichtegradientenzentrifugation
  • analytische Proteinbiochemie (ELISA, alle Elektrophorese- und Blot-Verfahren)
  • präparative Proteinbiochemie (Säulenchromatographie) 
  • Langzeit-Zellkultur

Aktuelle Publikationen

Müller S, Krüger B, Lange F, Bock CN, Nizze H, Glass Ä, Ibrahim SM, Jaster R (2014)
The mtDNA nt7778 G/T polymorphism augments formation of lymphocytic foci but does not aggravate cerulein-induced acute pancreatitis in mice.
PLoS ONE 9(7): e102266.

Weitzel JM, Vernunft A, Krüger B, Plinski C, Viergutz T (2014)
Inactivation of the LOX-1 pathway promotes the Golgi Apparatus during cell differentiation of mural granulosa cells.
J Cell Physiol 229(12): 1946-51

Müller S, Kaiser H, Krüger B, Fitzner B, Lange F, Bock, C, Nizze H, Ibrahim SM, Füllen G, Wolkenhauer O, Jaster R (2014)
Age-dependent effects of UCP2 deficiency of experimental acute pancreatitis in mice.
PLoS ONE 9(4): e94494.

Weber H, Jonas L, Wakileh M, Krüger B (2014).
Beneficial effect of the bioflavonoid quercetin on cholecystokinin-induced mitochondrial dysfunction in isolated rat pancreatic acinar cells.
Can J of Physiol and Pharmacol 92(3): 215-225.

Schick V, Scheiber JA, Mooren FC, Turi St., Ceyhan GO, Schnekenburger J, Sendler M, Schwaiger T, Omercevic A, van den Brandt C, Fluhr G, Domschke W, Krüger B, Mayerle J, Lerch MM (2014)
Effect of magnesium supplementation and depletion on the onset and course of acute experimental pancreatitis.
GUT 63(9): 1469-80.

Weitzel JM, Vernunft A, Krüger B, Plinski C, Viergutz T (2014)
LOX-1 regulates estrogenesis via intracellular calcium release from bovine granulosa cells.
Cytometry A 85(1): 88-93.

Sendler M, Dummer A, Weiss F, Krüger B, Wartmann T, Scharffetter-Kochanek K, van Rooijen N, Malla S, Aghdassi A, Halangk W, Lerch M, Mayerle J (2013)
Tumour necrosis factor alpha secretion induces protease activation and acinar cell necrosis in acute experimental pancreatitis in mice.
GUT 62(3): 430-439.

Malo A, Krüger B, Göke B, Kubisch Ch (2013)
4-phenylbutyric acid reduces endoplasmic reticulum stress, trypsin activation and acinar cell apoptosis while increasing secretion in rat pancreatic acini.
Pancreas 42(1): 92-101.

Buchveröffentlichung

Geissler K & Krüger B (2013)
Influence of pancreatic acinar cell necrosis on stellate cell proliferation in vitro.
Proceedings of the ICCB 2012. Ed. H.F.Carvalho and P. Gama.
Medimond, Bologna 2013 (ISBN 978-88-7587-672-2). pp. 131-137.

Online-Veröffentlichung

Krüger B & Lerch MM (2012)
Quantitation and visualization of protease activation in pancreatic acini.

The Pancreapedia: Exocrine Pancreas Knowledge Base, DOI: 10.3998/panc.2012.3